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Ensure that the NTFS permissions for the web.config file are correct and allow access to the Web server's machine account.
Check the event logs to see if any additional information was logged.
Verify the permissions for the DLL.
Install the .NET Extensibility feature if the request is mapped to a managed handler.
Create a tracing rule to track failed requests for this HTTP status code. For more information about creating a tracing rule for failed requests, click
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